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White Papers and Videos

Using NanoDrop Spectrophotometers in NMR, XRC, and Cryo-EM Workflows for Structural Biology

White Paper

Structural biology methods such as nuclear magnetic resonance (NMR), X-ray crystallography (XRC), and cryogenic electron microscopy (cryo-EM) are staples for drug discovery, virus research, and many other applications. Determining protein structure with structural biology methods grants a better understanding of molecular mechanisms as protein functionality can be related to structure.

This whitepaper from Thermo Fisher Scientific describes how to integrate NanoDrop spectrophotometers into NMR, XRC, and cryo-EM workflows to verify that protein samples remain pure and concentrated throughout structural biology processes.

Application Note - Trimer Phosphoramidites

White Paper

This application note from Glen Research presents three use cases for trimer phosphoramidites — trinucleotide reagents used to synthesize oligonucleotides based on amino acid codons rather than individual nucleotides — which allow researchers to create defined sequence libraries while avoiding stop codons and amino acid redundancy.

Cases include a screening method for protein affinity agents developed by the Regan Group at Yale University, an investigation of antibody regions by the Sidhu Group at the University of Toronto, and a description of a directed evolution method called compartmentalized partnered replication by the Ellington Group at the University of Texas at Austin.

Reagent Manufacturing for a Novel CRISPR-Based Diagnostic SARS CoV-2 Test

White Paper

This whitepaper from Millipore Sigma presents a case in which the business partnered with Mammoth Biosciences to manufacture reagents for a turnkey workstation to run a CRISPR-based SARS-CoV-2 molecular assay on automated liquid handling equipment.

The Importance of FGFR as a Biomarker in Bladder Cancer

Video

Certain FGFR alterations are key drivers of tumor growth in urothelial cancer, which is the sixth most common type of cancer in the US and is associated with poor prognosis. Erdafitinib has been approved for the treatment of urothelial carcinoma patients whose tumors harbor specific FGFR mutations or fusions. QIAGEN’s therascreen FGFR RGQ RT-PCR Kit was developed and FDA-approved as a companion diagnostic to identify such FGFR alterations.

In this discussion, expert panelists will examine the biological relevance and clinical significance of FGFRalterations and how FGFR testing can be leveraged to identify eligible patients.

Speakers include:

Dr. Arlene O. Siefker-Radtke, MDProfessor, Department of Genitourinary Medical Oncology University of Texas MD Anderson Cancer Center

Dr. Anjen Chenn, MD, PhDDiscipline Director, Molecular Oncology Labcorp

Dr. Neal D. Shore, MD, FACS Director, CPI Carolina Urologic Research Center

Advancing Research with the SARS-CoV-2 LC-MS Kit (RUO)

White Paper

Detection and quantification of SARS-CoV-2 are important in clinical research to understand the impact of SARS-CoV-2?and the significance of prolonged viral shedding.

This whitepaper from Waters Corporation demonstrates the capabilities of the SARS-Cov-2 LC-MS Kit (RUO) to directly detect and quantify low levels of SARS-CoV-2 signature nucleocapsid peptides without the need to amplify the target analytes — as required for PCR — and with the ability to measure multiple analytes at a time.

Digital PCR Principles and Advantages

Video

The detection of a rare allele or mutant sequence lost in a dense background of wild type sequences is a challenge in both research and diagnostic applications of molecular biology. This situation is often encountered in oncology when trying to detect tumor-derived mutant sequences, present in very small amounts in liquid biopsies.

This video from Stilla describes the principles behind digital PCR and the technology’s advantages over traditional bulk qPCR, focusing on liquid biopsy to detect tumor-derived DNA as an example.

Design and Optimize High Multiplexing Digital PCR Assays with the Naica System

Video

This on-demand webinar from Stilla discusses considerations for designing and optimizing digital PCR assays to maximize the potential of PCR instruments and presents data on SNP detection in liquid biopsy samples obtained using the Naica dPCR instrument.

Topics covered in the webinar include considerations for dye selection, inter-assay dimer concerns, and comparing singleplex with multiplex data to ensure assay compatibility. The second part of the webinar focuses on highly multiplexed dPCR assays using the Stilla Naica system for Crystal Digital PCR. The Naica system is a digital PCR solution with flexible sample throughput and highly sensitive detection and quantification of target nucleic acids.

Podcast: "A Coming of Age for Digital PCR"

Video

Quantitative PCR (qPCR) is a decades-old, established technique for quantifying DNA and RNA. But in recent years, digital PCR has emerged, providing advantages with sensitivity and ease of analysis.

This podcast from Stilla discusses the pros and cons of digital PCR and how it compares with qPCR and next-generation sequencing as a research and discovery method.

History of Digital PCR: (R)Evolution in Genetic Testing & Analysis

Video

The power of digital PCR lays in the combination of limiting dilution, microfluidics, massive parallelization of individual nanoliter-scaled PCR reactions, and absolute quantification with a binary readout.

This on-demand webinar from Stilla explains the principles and advantages of digital PCR (dPCR), the major dPCR platforms, and the array of possible applications for the technology, including a three-color target multiplex HIV quantification assay using the Naica System.

Accurate LC-MS Bioanalytical Quantification of Itaconic Acid Using the Automated Andrew+ Pipetting Robot for Ostro Sample Preparation

White Paper

This application note from Waters Corporation demonstrates automated sample preparation and protein precipitation extraction of itaconic acid from human plasma for liquid chromatography-mass spectrometry analysis and quantification using the Andrew+ Pipetting Robot with the Waters Ostro 96-well Plate. When compared to a manual workflow, the Andrew+ had better recoveries and improved RSDs, meeting recommended performance criteria for bioanalytical quantitation assays.

Automated Preparation and LC-MS/MS Analysis of Methylmalonic Acid in Serum Using the Andrew+ Pipetting Robot and Xevo TQ-S Micro

White Paper

The analysis of methylmalonic acid (MMA) can be used to determine the status of vitamin B12 serum levels. MMA is a preferable indicator to vitamin B12 due to the possibility of normal or high vitamin B12 serum levels even in deficient individuals. The use of liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis for research into this biomarker is ideal for high throughput applications.

This application note from Waters Corporation demonstrates the automated extraction of MMA from serum employing the Ostro Plate for protein precipitation and phospholipid removal on the Andrew+ Pipetting Robot. The development of the MMA Andrew+ protocol takes advantage of the existing MMA workflow and LC-MS analytical method which allows for the adoption of high throughput sample preparation.

Potential Clinical Utility of Measuring SARS-CoV-2-Specific T-Cell Responses

Video

In this webinar, Dr. Alessandro Sette will review his team’s research on T-cell responses to SARS-CoV-2 over the past year. Their early work predicted potential targets of T-cell immunity and demonstrated the immune responses of early convalescents. Subsequent studies analyzed correlates of immunity in the acute phase of disease, suggesting a key role for innate immunity and early T-cell responses correlating with less?severe disease outcomes. Parallel studies?highlighted the phenomenon of cross-reactivity between SARS-CoV-2 and common cold coronaviruses at the level of T-cell recognition and hypothesized a potential role of this cross-reactive immunity in the modulation of disease severity and responsiveness to vaccination. The team’s most recent studies characterized the duration of immune responses following natural infection, defined the epitope repertoire for SARS-CoV-2-reactive T cells, and analyzed the impact of variant-associated mutations on T-cell recognition.

Prenatal Clinical Samples Enable Development of QC Reference Materials for Prenatal and Preimplantation Genetic Testing

White Paper

Advancing prenatal testing requires an adequate supply of reference materials with clinical and genetic diversity. Using patient remnant samples for assay controls is not a viable or scalable option. Development of assays and routine testing for trisomies and microdeletions requires highly characterized reference materials to ensure the quality of testing.

This poster from SeraCare describes the development and clinical performance of reference materials for prenatal and preimplantation testing that appear suitable for a range of currently available PGT-A and NIPT methods. The reference material methodology can be applied to emerging genetic testing methodologies designed for rare disease testing.

A Novel Whole Genome Amplification (WGA) Method for the Detection of Chromosomal Abnormalities

White Paper

Improving the outcomes of in vitro fertilization treatment requires better methods to predict the risk of miscarriage. One of the factors affecting the success of pregnancy is embryonic aneuploidy. Preimplantation genetic testing is a way to screen or test embryos generated in the lab for the presence or absence of genetic alterations that can reduce the embryo’s viability or affect the health of the fetus.

This poster from SeraCare presents data on newly developed reference materials for preimplantation genetic testing for aneuploidies and demonstrates the materials’ performance using primary template-directed amplification for whole genome amplification.

Introducing Professional Assay Services - RNAscope ISH Experts in Tissue Expression Analysis

Video

This webinar from ACD presents use cases in immuno-oncology, cell and gene therapy, neuroscience, single-cell RNAseq validation, biomarker validation, and others demonstrating applications for RNAscope in situ hybridization services. Uses include spatial visualization of cellular heterogeneity in tumors, biomarker validation, biodistribution analysis of adeno-associated virus vector and transgene expression, CAR-T cell tumor infiltration assays, and others.

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